We present an optimized dissociation protocol for preparing high-quality skin cell suspensions for in-depth single-cell RNA-sequencing (scRNA-seq) analysis of fresh and cultured human skin.Our protocol enabled the isolation of a consistently high number of highly viable skin cells from small freshly dissociated punch skin biopsies, which we use for
Abstract ETS2 repressor factor (ERF) is a member of the ETS family of transcriptional repressors downstream of ERK.Although germline truncated variants in ERF have been identified Blackboard in individuals with Noonan-like syndrome with or without craniosynostosis, the clinical spectrum of ERF variant-positive individuals and the functional charact
The production of food-grade salt from crude solar salt has been examined through a techno-economic evaluation.This study aimed to investigate a salt factory to analyze its technical and economic aspects to determine the precise parameters for improving the quality of food-grade salt.The primary process of this factory involves grinding, washing, d
L-Methylfolate This study proposes a sub-kHz clock generator by the room light illumination designed on-chip CMOS LSI chips.It generates a clock from a flicker in room light by receiving on-chip integrated photovoltaic cell, and extracts by low-pass filters, a comparator, Schmidt trigger and D-flip-flop to shape to digital signal.The system impleme